Plant growth
The last lesson showed how water and minerals reach the leaves; here we see how a plant grows, which hormones control it and how it gets nitrogen.
A seedling gains a few centimetres every week — but only near its tips.
Growth = permanent, irreversible increase in the bulk of an organism. In plants it occurs in meristems (indeterminate).
In the figure : Meristems, phases and the sigmoid curve.
Rule 1 : Meristems and phases of growth — Apical meristem (tips of root and shoot) = length; intercalary (base of internodes) = internode length; lateral (vascular cambium) = girth. Root and stem growth is indeterminate; leaves, flowers, fruits are determinate. Three phases: (A) cell division (thin walls, large nucleus; slow = lag), (B) cell enlargement (vacuole, turgid, new wall; fast = log), (C) maturation (specialisation; steady = stationary).
- Easy : Apical = length; cambium = girth.
- Medium : Lag slow, log fast.
- Exam-type (CET) : Leaves: determinate.
Rule 2 : Growth rate, types and conditions — AGR (absolute growth rate) = total growth per unit time; RGR = growth relative to the initial size. Example (own): a 5 cm² leaf grows 5 cm²/day ⇒ RGR 100%; a 50 cm² leaf grows the same ⇒ RGR 10%. Arithmetic growth: constant rate (2, 4, 6, 8 cm), one daughter cell divides, the other matures; Lt = L0 + rt; linear graph. Geometric: both daughters divide; W1 = W0·e^(rt); exponential graph; together they give the sigmoid (S) curve; total time of all phases = grand period. Conditions: water, nutrients, 25–35 °C, O₂, light, gravity, hormones. Measured with auxanometer, crescograph (J. C. Bose; 10,000×).
- Easy : RGR is higher in a small leaf.
- Medium : Arithmetic = linear.
- Exam-type (CET) : Sigmoid = lag + log + stationary.
Apical = height; lateral = girth.
‘All organs grow indefinitely’ is wrong. Trap: ‘growth is fastest in lag phase’ is wrong.
Memory clue : "LLS = lag, log, stationary — the S-curve".
Spot the difference
| Type | Arithmetic | Geometric |
|---|---|---|
| Division | One daughter | Both daughters |
| Graph | Linear | Exponential |
Practice questions
- 1Types of meristem?
- 2What increases girth?
- 3Log phase?
- 4RGR is?
- 5A 10 cm² leaf adds 5 cm²; RGR?
- 6Whose crescograph?
- 7Grand period?
- 8Determinate growth in?
Show answers (try them yourself first)
- 1Apical, intercalary, lateral.
- 2Vascular cambium.
- 3Fast growth.
- 4Growth relative to initial size.
- 550%.
- 6J. C. Bose.
- 7Total time of all phases.
- 8Leaves, flowers, fruits.
Differentiation and plasticity
Clay fired into a brick can't return to clay; a cell is similar.
Differentiation = permanent change in a cell for specialisation. Plasticity = ability to form different structures in different conditions.
In the figure : Differentiation, dedifferentiation and redifferentiation.
Rule 1 : The three terms — Differentiation: maturation of cells from root/shoot tips; permanent structural and functional change; division ability is lost; e.g. parenchyma of hydrophytes with large air spaces. Dedifferentiation: a living mature cell regains division (becomes meristematic); e.g. interfascicular cambium and cork cambium from parenchyma. Redifferentiation: cells so formed again lose division and take a function; e.g. secondary xylem.
- Easy : Dedifferentiation = divides again.
- Medium : Cork cambium = example.
- Exam-type (CET) : Redifferentiation = secondary xylem.
Rule 2 : Development and plasticity — Development = ordered changes from germination to death/senescence: growth + morphogenesis + maturation + senescence. Plasticity: a plant forms different structures in response to internal/external cues. Heterophylly: different leaf shapes on one plant — juvenile vs adult (cotton, coriander, larkspur: intrinsic); aquatic vs terrestrial (Ranunculus: environmental).
- Easy : Development includes growth and maturity.
- Medium : Cotton: intrinsic.
- Exam-type (CET) : Ranunculus: environmental.
De-diff = divide again; re-diff = settle to a role again.
‘A mature cell can never divide’ is wrong (dedifferentiation). Trap: ‘heterophylly = different plants’ is wrong.
Memory clue : "D–D–R in order".
Spot the difference
| Term | Division ability |
|---|---|
| Differentiation | Lost |
| Dedifferentiation | Regained |
| Redifferentiation | Lost again |
Practice questions
- 1What is differentiation?
- 2Cork cambium arises from?
- 3Interfascicular cambium arises from?
- 4Example of redifferentiation?
- 5Heterophylly example?
- 6Environmental heterophylly in?
- 7Development includes?
- 8Define plasticity.
Show answers (try them yourself first)
- 1Permanent change for specialisation.
- 2Cortex cells by dedifferentiation.
- 3Parenchyma.
- 4Secondary xylem.
- 5Cotton / coriander.
- 6Ranunculus.
- 7Growth, morphogenesis, maturation, senescence.
- 8Changing form with conditions.
Auxin and gibberellin
A plant near a window bends to the light — thanks to auxin.
Plant hormones = organic substances made by plants; act away from the site of production, in very small amounts (Thimann–Pincus 1948).
In the figure : Coleoptile and auxin; uses of gibberellin.
Rule 1 : Auxin — Discovery: Darwin 1886 (bending to light), Boysen-Jensen 1910 (gelatin plate), Paal 1919, Went 1928 (Avena coleoptile + agar block). Natural auxin = IAA (from tryptophan); synthetic: IBA, NAA, 2,4-D. Made in growing tips; basipetal transport. Functions: cell elongation; apical dominance; callus in tissue culture; rooting; prevents fruit drop; flowering in litchi/pineapple; breaks seed dormancy. 2,4-D is a weedicide; Agent Orange = 2,4-D + 2,4,5-T.
- Easy : IAA = natural auxin.
- Medium : Went = 1928.
- Exam-type (CET) : Apical dominance = auxin.
Rule 2 : Gibberellin — Discovery: Kurosawa 1926 — fungus Gibberella fujikuroi caused bakane (over-tall) disease of rice; Yabuta–Sumiki 1938 crystallised it. Commonest GA₃; 150+ kinds; made in young leaves, seeds, roots, tips; non-polar transport. Functions: internode elongation, dwarf → tall (maize), bolting (beet, cabbage), parthenocarpy (tomato, apple), larger grape bunches, germination of barley/wheat (α-amylase), breaks dormancy, replaces vernalization; inhibits root growth.
- Easy : GA₃ = gibberellic acid.
- Medium : Bakane = rice.
- Exam-type (CET) : Bolting = beet, cabbage.
Auxin = cell stretch & light bending; GA = gets taller.
‘Auxin moves acropetally’ is wrong (basipetal). Trap: ‘GA promotes root growth’ is wrong.
Memory clue : "IAA = I am auxin; GA = gets taller".
Spot the difference
| Hormone | Main role |
|---|---|
| Auxin | Cell elongation, apical dominance |
| GA | Internode length, bolting |
Practice questions
- 1First plant hormone discovered?
- 2Went used which plant?
- 3Natural auxin?
- 4Apical dominance is due to?
- 5Use of 2,4-D?
- 6Bakane disease?
- 7One use of GA?
- 8Parthenocarpy induced by?
Show answers (try them yourself first)
- 1Auxin.
- 2Avena coleoptile.
- 3IAA.
- 4Auxin.
- 5Weedicide.
- 6Gibberella → over-growth of rice.
- 7Dwarf → tall / grape bunches.
- 8GA / auxin.
Cytokinin, ethylene and ABA
Raw mangoes ripen near a ripe banana — due to ethylene gas.
Cytokinin = cell-division hormone. Ethylene = the only gaseous hormone. ABA = growth-inhibiting stress hormone.
In the figure : Three hormones and their roles.
Rule 1 : Cytokinin — Skoog–Miller 1954 — tobacco callus grew with coconut milk and old DNA; kinetin (6-furfuryl amino purine). First natural = zeatin (from young maize, Letham). Functions: cell division and enlargement; high cytokinin : auxin = shoots, low = roots; growth of lateral buds (reverses apical dominance); delays senescence and abscission; seed germination; RNA synthesis; interfascicular cambium.
- Easy : Kinetin = 1954.
- Medium : Zeatin = natural.
- Exam-type (CET) : High CK = shoots.
Rule 2 : Ethylene and ABA — Ethylene: Denny 1924 (ripening), Gane 1934 (plants make it), Crocker 1930. Ethephon (2-chloroethyl phosphonic acid) releases ethylene in water. Functions: ripens fruit (banana, apple, mango), abscission layer, lateral roots, breaks dormancy, epinasty, flowering in pineapple, degreening of banana/citrus. ABA: Addicott 1961–65 (abscisin; cotton boll fall), Wareing 1963 (dormin, Acer buds); 15-carbon sesquiterpenoid. Functions: dormancy, abscission, senescence, inhibits cell division, K⁺ efflux from guard cells ⇒ stomata close (antitranspirant); inhibits flowering in long-day plants.
- Easy : Ethylene = gas; ripens fruit.
- Medium : ABA = antitranspirant.
- Exam-type (CET) : Ethephon = source of ethylene.
CK = cell division; ethylene = ripening; ABA = brake.
‘Ethylene is a liquid hormone’ is wrong (gas). Trap: ‘ABA opens stomata’ is wrong.
Memory clue : "Promoters = A-G-C; inhibitors = E-A".
Spot the difference
| Hormone | Effect |
|---|---|
| Cytokinin | Cell division; delays senescence |
| Ethylene | Ripening; abscission |
| ABA | Dormancy; stomata close |
Practice questions
- 1Who discovered kinetin?
- 2First natural cytokinin?
- 3Low CK : auxin gives?
- 4Only gaseous hormone?
- 5What is ethephon?
- 6Degreening is caused by?
- 7Another name of ABA?
- 8Which hormone is antitranspirant?
Show answers (try them yourself first)
- 1Skoog and Miller (1954).
- 2Zeatin.
- 3Roots.
- 4Ethylene.
- 5A source of ethylene.
- 6Ethylene.
- 7Dormin / abscisin.
- 8ABA.
Photoperiodism and vernalization
Some flowers open only in winter and some only in summer — due to length of daylight.
Photoperiodism = effect of relative day/night length on flowering. Vernalization = acceleration of flowering ability by chilling.
In the figure : SDP, LDP, DNP and phytochrome.
Photoperiodism and vernalization
In the app you can move this figure with your finger.
Open in the appRule 1 : Photoperiodism — Garner–Allard 1920 (soybean; Maryland Mammoth tobacco) — not temperature, nutrition or moisture but light duration decides flowering. SDP (long night; day shorter than critical photoperiod): Dahlia, Aster, Chrysanthemum, Xanthium; a flash in the night stops flowering. LDP (short night): pea, radish, beet, cabbage, spinach, wheat. DNP: tomato, cotton, sunflower, maize. Leaf = receptor (Knoff 1934); florigen moves through phloem. Phytochrome: Pr (660 nm red) ⇄ Pfr (730 nm far-red); Hendricks–Borthwick 1952; Pfr accumulates by day (inhibits SDP, promotes LDP); at night Pfr → Pr (promotes SDP).
- Easy : SDP = long night.
- Medium : Phytochrome = Pr ⇄ Pfr.
- Exam-type (CET) : Leaf = receptor.
Rule 2 : Vernalization — Klippart noticed winter vs spring wheat; Lysenko 1928 coined the term. Method: seeds/seedlings chilled at 1–6 °C for one to one-and-a-half months. Winter wheat without chilling flowers only the next year. Site = shoot apical meristem; chemical stimulus vernalin (Melchers) — transferred by grafting (Melcher 1939). Reversal by heat = devernalization. Advantages: earlier flowering, shorter juvenile phase, crops in regions without cold.
- Easy : 1–6 °C.
- Medium : Devernalization = heat.
- Exam-type (CET) : Site = apical meristem.
SDP = long dark; LDP = short dark.
‘SDP needs long days’ is wrong (long nights). Trap: ‘Pfr accumulates at night’ is wrong (by day).
Memory clue : "Red makes Pfr; far-red makes Pr".
Spot the difference
| Type | Night | Example |
|---|---|---|
| SDP | Long | Chrysanthemum |
| LDP | Short | Pea |
| DNP | Independent | Tomato |
Practice questions
- 1Who coined photoperiodism?
- 2SDP example?
- 3LDP example?
- 4Receptor organ?
- 5Florigen travels through?
- 6Vernalization temperature?
- 7Who coined vernalization?
- 8Devernalization is?
Show answers (try them yourself first)
- 1Garner and Allard.
- 2Dahlia / Chrysanthemum.
- 3Pea / wheat.
- 4Leaf.
- 5Phloem.
- 61–6 °C.
- 7Lysenko (1928).
- 8Reversal by high temperature.
Mineral nutrition
Water alone doesn't feed a field; fertiliser adds the minerals.
An essential element = one without which the plant cannot complete its life cycle. Chlorosis = loss of chlorophyll; yellow leaves.
In the figure : Macro/micro elements and deficiency signs.
Rule 1 : Classification and deficiency symptoms — About 36–40 elements are used; as ions, through roots. C, H, O come from air and water; N from soil (mineral and non-mineral). Macro (large amounts: structure/nutrition): C, H, O, N, P, K, Ca, Mg, S, Fe; micro (traces: catalytic, co-factors): Zn, Cu, Mn, B, Mo, Cl. Criteria of essentiality (Arnon–Stout 1939): indispensable for normal growth and reproduction; specific role; cannot be replaced by another. Liebig: law of minimum. Symptoms: stunting, chlorosis, necrosis, mottling, abscission. Mobile (N, Mg, K): old leaves first; immobile (S, Ca): young leaves first.
- Easy : Mobile = old leaves first.
- Medium : Immobile = S, Ca.
- Exam-type (CET) : Liebig = law of minimum.
Rule 2 : Roles of some elements; absorption — N: proteins, nucleic acids, chlorophyll → deficiency: stunting, chlorosis. P: membranes, ATP, nucleic acids. K: stomata, turgor, enzymes. Ca: middle lamella (Ca pectate). Mg: chlorophyll. S: proteins, vitamins. Zn: auxin synthesis; B: brown heart; Mo: N metabolism; Cu: die-back; Cl: O₂ evolution. Absorption (independent of water): passive (diffusion, ion-exchange, mass flow, Donnan equilibrium) and active (against gradient, ATP; carrier hypothesis — Hoagland–Davis 1923). Without O₂ active uptake drops; transport xylem → leaves → phloem. A concentration cutting dry weight by 10% = toxic.
- Easy : Mg = chlorophyll.
- Medium : Active = ATP.
- Exam-type (CET) : Boron = brown heart.
N, Mg, K show in old leaves; S, Ca in young leaves.
‘Ca moves from old to young leaves’ is wrong. Trap: ‘minerals are absorbed only with water’ is wrong.
Memory clue : "C-HOPKNS CaFe Mg = macro".
Spot the difference
| Element | Role | Deficiency |
|---|---|---|
| N | Proteins | Chlorosis |
| Mg | Chlorophyll | Chlorosis |
| B | Ca uptake | Brown heart |
Practice questions
- 1Macro example?
- 2Micro example?
- 3Chlorosis means?
- 4Liebig's law?
- 5A mobile element?
- 6Where does Ca function?
- 7Donnan equilibrium is?
- 8Active absorption needs?
Show answers (try them yourself first)
- 1N / P / K.
- 2Zn / Cu / Mn.
- 3Yellowing of leaves.
- 4Law of minimum.
- 5N / K / Mg.
- 6Middle lamella.
- 7Passive absorption.
- 8ATP / energy.
Nitrogen cycle
Soil after gram or tur becomes fertile for the next crop — thanks to root nodules.
Nitrogen fixation = conversion of atmospheric N₂ into salts that plants can use.
In the figure : Stages of the nitrogen cycle.
Nitrogen cycle
In the app you can move this figure with your finger.
Open in the appRule 1 : Nitrogen fixation — N₂ has an N≡N triple bond — inert. Physical: lightning, N₂ + O₂ → 2NO → 2NO₂ → rain gives HNO₂ + HNO₃ → with Ca/K salts in soil forms nitrates. Industrial: Haber–Bosch — N₂ + 3H₂ → 2NH₃, 450 °C, 200 atm; then urea. Biological (~70%): diazotrophs — Rhizobium (nodules on legumes; symbiotic), Azotobacter (free-living), cyanobacteria (heterocysts). Nitrogenase: N₂ + 8H⁺ + 8e⁻ + 16 ATP → 2NH₃ + H₂ — energy-costly.
- Easy : Haber–Bosch = 450 °C, 200 atm.
- Medium : Rhizobium = legumes.
- Exam-type (CET) : Biological = ~70%.
Rule 2 : The other steps of the cycle — Ammonification: fungi, actinomycetes and bacteria decompose dead plants/animals: protein → amino acids → NH₃. Nitrification: Nitrosomonas NH₃ → NO₂⁻; Nitrobacter NO₂⁻ → NO₃⁻. Assimilation: roots take NO₃⁻ → NH₃ → amino acids → proteins; animals get it by eating plants. Denitrification: Pseudomonas, Thiobacillus — NO₃⁻ → N₂, back to atmosphere. Soil nitrogen is a limiting nutrient in agriculture.
- Easy : Nitrosomonas: NH₃ → NO₂⁻.
- Medium : Nitrobacter: NO₂⁻ → NO₃⁻.
- Exam-type (CET) : Denitrification → N₂.
Nitrosomonas makes nitrite; Nitrobacter takes it to nitrate.
‘Rhizobium is free-living’ is wrong (symbiotic). Trap: ‘denitrification increases nitrate’ is wrong.
Memory clue : "A-N-A-D steps".
Spot the difference
| Process | Organism | Result |
|---|---|---|
| Fixation | Rhizobium | N₂ → NH₃ |
| Nitrification | Nitrosomonas / Nitrobacter | NO₃⁻ |
| Denitrification | Pseudomonas | N₂ |
Practice questions
- 1Why is N₂ inert?
- 2Haber–Bosch conditions?
- 3Rhizobium lives in?
- 4Heterocysts occur in?
- 5Ammonification is?
- 6Function of Nitrosomonas?
- 7Denitrification gives?
- 8Biological fixation %?
Show answers (try them yourself first)
- 1Triple bond.
- 2450 °C, 200 atm.
- 3Root nodules of legumes.
- 4Cyanobacteria.
- 5Dead matter → NH₃.
- 6NH₃ → NO₂⁻.
- 7N₂.
- 8~70%.
Quick revision of the whole lesson
| Concept | Key fact |
|---|---|
| Growth | Lag → log → stationary; AGR/RGR |
| Differentiation | De-diff = divides again |
| Auxin / GA | IAA (Went 1928); GA₃ (Kurosawa 1926) |
| CK / C₂H₄ / ABA | Kinetin 1954; ripening; stomata close |
| Photoperiod | SDP long night; vernalization 1–6 °C |
| Minerals | Mobile N, Mg, K; immobile S, Ca |
| N cycle | Fixation → nitrification → denitrification |
🎯 Most important points for the exam
- Meristems and phases.
- Dedifferentiation, plasticity.
- Auxin and gibberellin.
- Cytokinin, ethylene, ABA.
- SDP/LDP, phytochrome, vernalization.
- Macro/micro, deficiency signs.
- Nitrogen cycle.
Understand the concept → draw the figure → memorise the key points → solve questions yourself.