Cell, history and cell theory
This deepens the basic cell structure of Std 9–10 (cell wall, nucleus, mitochondria, chloroplast) to the structure and function of every organelle.
A wall is built brick by brick and a body is built cell by cell; the smallest cells are invisible to the eye while an ostrich egg is the largest example.
Cell = structural and functional unit of life, capable of independent existence. Cell theory: Schleiden (1838) and Schwann (1839) stated that plants and animals are made of cells; Virchow (1855): new cells arise from pre-existing cells by division.
In the figure : Top: timeline of the cell theory; below: cell sizes on a log scale.
Rule 1 : Postulates of the modern cell theory — All organisms consist of cells; new cells arise from old; the cell is the unit of structure and function; total cell activity gives organism activity; cells transform energy; cells contain DNA and RNA.
- Easy : ‘Omnis cellula e cellula’ = every cell from a cell (Virchow).
- Medium : The word cell was used by Robert Hooke (1665) in ‘Micrographia’.
- Exam-type (CET) : Totipotency = ability of a cell to form a whole organism (embryonic stem cells).
Rule 2 : Cell sizes and microscopes — Smallest: Mycoplasma 0.3 µm; bacteria 3–5 µm; largest: ostrich egg (~15 cm); longest: nerve cell. Light microscope ~1000×, electron microscope ~500,000×. Purkinje–Mohl: protoplasm; Robert Brown: nucleus; Golgi; Porter: ER; de Duve: lysosome.
- Easy : Cell size is usually in µm; 1 µm = 10⁻⁶ m.
- Medium : An electron microscope shows cell organelles.
- Exam-type (CET) : Virchow filled the gap (how are new cells formed?).
Hooke 1665 (cell), Schleiden 1838, Schwann 1839, Virchow 1855; smallest Mycoplasma.
‘Virchow coined cell’ is wrong (Hooke). Trap: swapping Schleiden (plants) and Schwann (animals) is wrong.
Memory clue : "HSSV: Hooke → Schleiden → Schwann → Virchow".
Spot the difference
| Scientist | Year | Contribution |
|---|---|---|
| Hooke | 1665 | Term cell |
| Schleiden | 1838 | Plants = cells |
| Schwann | 1839 | Animals = cells |
| Virchow | 1855 | Cell from cell |
Practice questions
- 1What is a cell?
- 2Who used the term cell?
- 3Who proposed the cell theory?
- 4Contribution of Virchow?
- 5Largest and smallest cells?
- 6What is totipotency?
- 7Three postulates of modern cell theory.
- 8Advantage of an electron microscope?
Show answers (try them yourself first)
- 1Structural and functional unit of life.
- 2Robert Hooke (1665).
- 3Schleiden and Schwann.
- 4New cells arise from pre-existing cells.
- 5Ostrich egg; Mycoplasma.
- 6Capacity of a cell to give a whole organism.
- 7Organisms are cellular; cells from cells; cell = unit.
- 8Very high magnification; shows cell interior.
Prokaryotic cell
The bacteria that set curd have no nuclear membrane; their DNA lies loose in the cytoplasm.
Prokaryotic cell = a cell without a definite nucleus or membrane-bound organelles (bacteria, cyanobacteria). The cell envelope has three layers: glycocalyx + cell wall + plasma membrane.
In the figure : Bacterial cell: cell envelope, nucleoid, plasmid, 70S ribosomes, flagellum.
Prokaryotic cell
In the app you can move this figure with your finger.
Open in the appRule 1 : Cell envelope and motility — Glycocalyx = slime layer (loose) or capsule (tough). Cell wall: peptidoglycan (Gram +ve), murein (Gram −ve) – mechanical strength. Plasma membrane = phospholipid bilayer. Motility by flagella; basal body (motor) + filament + hook. Pili = contact; fimbriae = clinging. Gram staining: Hans Christian Gram.
- Easy : Mesosome = membrane infolding; wall formation, respiration, DNA replication.
- Medium : Cyanobacteria have chromatophores (photosynthetic pigments).
- Exam-type (CET) : The basal body is often called the smallest motor in the world.
Rule 2 : Interior: DNA, ribosomes, plasmids — Genetic material = single circular DNA (nucleoid), no histones so no chromatin; attached to mesosome. Plasmid = small circular DNA: F-plasmid (reproduction), R-plasmid (antibiotic resistance). Ribosomes 70S = 50S + 30S. Cytoplasm has reserve granules (glycogen, cyanophycean starch, sulphur).
- Easy : A special DNA replication pattern: theta model.
- Medium : R-plasmid makes bacteria resist antibiotics.
- Exam-type (CET) : 70S refers to sedimentation rate (Svedberg).
Prokaryote: no nucleus, 70S ribosomes, circular DNA, plasmid, mesosome.
‘Prokaryotes have mitochondria’ is wrong. Trap: ‘histones present’ is wrong.
Memory clue : "Pro = Primitive, no nucleus; 70S = 50 + 30".
Spot the difference
| Point | Prokaryote | Eukaryote |
|---|---|---|
| Nuclear membrane | Absent | Present |
| Ribosomes | 70S | 80S |
| Membrane organelles | Absent | Present |
Practice questions
- 1What is a prokaryotic cell?
- 2Three layers of the cell envelope.
- 3Who developed Gram staining?
- 4What is a plasmid?
- 5Functions of F and R plasmids.
- 6Function of mesosome.
- 7Difference between pili and fimbriae.
- 8Two differences between prokaryote and eukaryote.
Show answers (try them yourself first)
- 1A cell lacking nuclear membrane and membrane organelles.
- 2Glycocalyx, cell wall, plasma membrane.
- 3Hans Christian Gram.
- 4Small circular extra DNA in bacteria.
- 5F: reproduction; R: antibiotic resistance.
- 6Wall formation, respiration, DNA replication.
- 7Pili: intercellular communication; fimbriae: clinging.
- 8Nuclear membrane absent/present; ribosomes 70S/80S.
Cell wall and plasma membrane
A ripe fruit turns soft as pectin in its wall dissolves; the plasma membrane works like a fence, letting in what is needed and keeping out the rest.
Cell wall = rigid outer covering in plants, fungi and some protists. Plasma membrane = lipid bilayer + proteins; fluid mosaic model (Singer–Nicholson, 1972); selectively permeable.
In the figure : Top: fluid mosaic model; below: layers of a plant cell wall and transport.
Rule 1 : Cell wall — Middle lamella (pectin, Ca and Mg pectate; first, from the cell plate), primary wall (young cell, can grow), secondary wall (after growth stops; pits). Algae: cellulose, galactans, mannans, CaCO₃; other plants: hemicellulose, pectin, lignin; cutin, suberin, silica deposited. Plasmodesmata connect neighbouring cells.
- Easy : Suberin in root endodermis; cutin in leaf epidermis.
- Medium : Cellulose = rigidity; pectin = cementing material.
- Exam-type (CET) : Meristematic cells have only a primary wall.
Rule 2 : Plasma membrane and transport — Thickness about 75 Å; phospholipids are amphipathic (hydrophilic heads + hydrophobic tails) in a bilayer. Proteins: integral (span the bilayer, channels) and peripheral. Passive: simple diffusion, osmosis – no energy. Active: against the gradient, ATP (Na⁺/K⁺ pump). Polar molecules need carrier proteins.
- Easy : ‘Icebergs in a sea’: proteins move in the lipid sea (fluidity).
- Medium : Water channels = integral proteins.
- Exam-type (CET) : Endomembrane system: ER, Golgi, lysosomes, vacuoles, nuclear envelope; not mitochondria/chloroplasts.
Passive = no energy; active = ATP + against gradient; Singer–Nicholson 1972.
‘Animals have a cell wall’ is wrong. Trap: ‘proteins are fixed’ is wrong (fluid).
Memory clue : "ML-PW-SW: Middle → Primary → Secondary (outside to inside)".
Spot the difference
| Transport | Energy | Example |
|---|---|---|
| Simple diffusion | No | O₂, CO₂ |
| Osmosis | No | Water |
| Active transport | ATP | Na⁺/K⁺ pump |
Practice questions
- 1Who proposed the fluid mosaic model?
- 2What does amphipathic mean?
- 3Integral vs peripheral proteins.
- 4What forms the middle lamella?
- 5What are plasmodesmata?
- 6Active vs passive transport.
- 7Why does a ripe fruit soften?
- 8When is the secondary wall laid?
Show answers (try them yourself first)
- 1Singer and Nicholson (1972).
- 2One end water-loving, the other water-repelling.
- 3Integral span the membrane; peripheral lie on the surface.
- 4Pectin, Ca/Mg pectate.
- 5Cytoplasmic bridges between neighbouring cells.
- 6ATP, against gradient / no energy, along gradient.
- 7Pectin dissolves.
- 8After primary wall growth stops.
Cytoplasm, ER and Golgi complex
A factory receives raw material, makes goods, packs and dispatches them. In a cell the ER makes, the Golgi packs and vesicles dispatch.
Cytoplasm (cytosol) = the jelly-like material of the cell; ER = network of membranous tubules and sacs (cisternae); Golgi complex = stacks of cisternae – modifies and packs secretions.
In the figure : Nucleus → rough ER → vesicle → Golgi (cis → trans) → outside / lysosome.
Cytoplasm, ER and Golgi complex
In the app you can move this figure with your finger.
Open in the appRule 1 : Cytoplasm and ER — Cytosol holds water, sugars, amino acids, enzymes; cyclosis = streaming. ER runs from the nuclear envelope to the cell membrane; over 50% of cell membrane. Rough ER: ribosomes; secretory proteins, builds membrane. Smooth ER: lipids/steroids (adrenal, testes), detoxification (liver), Ca²⁺ store (muscle). ER absent: mature RBCs, ova.
- Easy : Cells making insulin have abundant rough ER.
- Medium : Smooth ER in liver = drug/alcohol detox.
- Exam-type (CET) : Cytoplasm = raw material + site of metabolism.
Rule 2 : Golgi complex — Stacks of cisternae (0.5–1 µm); cis (receiving) and trans (dispatching) faces; ER vesicles fuse at cis; secretions are modified (glycoproteins, glycolipids) and leave from trans as vesicles. Golgi also makes non-cellulose polysaccharides like pectin. Golgi cisternae are not connected like ER. Lysosomes arise from Golgi.
- Easy : Cis = near (toward ER); trans = far.
- Medium : Discovered by Camillo Golgi (1838).
- Exam-type (CET) : Cisternal maturation model: cisternae themselves mature from cis to trans.
Rough ER = proteins; smooth ER = lipids/detox; Golgi = modify and pack (cis → trans).
‘Smooth ER has ribosomes’ is wrong. Trap: ‘Golgi cisternae are connected like ER’ is wrong.
Memory clue : "RER = Ribosomes + Protein; SER = Steroids + Safe (detox)".
Spot the difference
| Organelle | Function | Key |
|---|---|---|
| Rough ER | Proteins | Ribosomes |
| Smooth ER | Lipids, detox | No ribosomes |
| Golgi | Modify, pack | cis/trans |
Practice questions
- 1What is cytosol?
- 2What is cyclosis?
- 3Which cells lack ER?
- 4Function of rough ER.
- 5Two functions of smooth ER.
- 6Explain cis and trans faces.
- 7Which form the endomembrane system?
- 8One difference between Golgi and ER.
Show answers (try them yourself first)
- 1The fluid part of the cytoplasm.
- 2Streaming movement of cytoplasm.
- 3Mature RBCs and ova.
- 4Synthesis and transport of proteins.
- 5Lipid/steroid synthesis; detox; Ca²⁺ storage.
- 6cis = receiving; trans = dispatching.
- 7ER, Golgi, lysosomes, vacuoles, nuclear envelope.
- 8ER cisternae are connected; Golgi's are not.
Lysosomes, vacuoles and microbodies
As a garbage truck breaks down waste, a lysosome digests old material in the cell.
Lysosome = membrane-bound vesicle with hydrolytic enzymes (act at acidic pH). Vacuole = sac bounded by a tonoplast holding cell sap. Microbodies = small membrane-bound sacs: sphaerosome, peroxisome, glyoxysome.
In the figure : Types of lysosomes, plus vacuoles and microbodies in brief.
Rule 1 : Lysosomes — Formed from Golgi; enzymes inactive (primary) → fuse with foreign material/old organelles to form active secondary lysosomes (heterophagic/autophagic). Residual body = undigested remains. Autophagic = ‘suicide bag’. Acrosome = lysosome (hyaluronidase). Metamorphosis, destroying cancer cells (T-lymphocytes). Tay-Sachs: lipase deficiency → fat accumulates, brain damaged.
- Easy : Amoeba food vacuole = intracellular digestion.
- Medium : Macrophages engulf bacteria and destroy them with lysosomes.
- Exam-type (CET) : Lysosomes do not digest their own membrane (protein shape).
Rule 2 : Vacuoles and microbodies — Vacuole: large in plant cells (central vacuole up to 90%); tonoplast; cell sap hypertonic; maintains turgor; pigments (petal colour); contractile vacuole in Paramoecium (excretion + osmoregulation). Microbodies: sphaerosome (fat storage, endosperm), peroxisome (makes and breaks H₂O₂; alcohol in liver), glyoxysome (fat → sugar in germinating seeds).
- Easy : Central vacuole stores water and salts.
- Medium : Glyoxysome = in germinating seeds, until photosynthesis begins.
- Exam-type (CET) : Tonoplast = vacuole membrane.
Lysosome = digestion + ‘suicide bag’; vacuole = turgor; peroxisome = H₂O₂; glyoxysome = fat → sugar.
‘Lysosome enzymes always active’ is wrong (initially inactive). Trap: ‘tonoplast = lysosome membrane’ is wrong.
Memory clue : "Lyso = lysis (break down); Tono = Tonic (vacuole)".
Spot the difference
| Organelle | Function | Example |
|---|---|---|
| Lysosome | Digestion | Macrophage |
| Vacuole | Turgor, storage | Central vacuole |
| Peroxisome | H₂O₂ breakdown | Liver |
Practice questions
- 1Where do lysosomes arise?
- 2Type of lysosomal enzymes?
- 3Why is it called a suicide bag?
- 4Acrosome is a modified ___?
- 5What is a tonoplast?
- 6Function of contractile vacuole.
- 7Function of peroxisome.
- 8Where are glyoxysomes found and what do they do?
Show answers (try them yourself first)
- 1From the Golgi complex.
- 2Hydrolases (amylase, protease, lipase).
- 3It digests the cell's own old organelles.
- 4Lysosome.
- 5Membrane around the vacuole.
- 6Excretion and osmoregulation.
- 7Forms H₂O₂ and converts it to water.
- 8Germinating seeds; convert fat to sugar.
Mitochondria, plastids and ribosomes
A phone battery stores and delivers energy; in a cell mitochondria deliver ATP and chloroplasts make food from sunlight.
Mitochondrion = double-membraned energy factory (cristae, oxysomes). Plastids = leucoplast, chromoplast, chloroplast. Ribosome = membrane-less protein factory; 70S (prokaryote) and 80S (eukaryote).
In the figure : Structure of mitochondrion and chloroplast; ribosome sizes and the endosymbiont theory.
Mitochondria, plastids and ribosomes
In the app you can move this figure with your finger.
Open in the appRule 1 : Mitochondria — Double membrane: outer (porin; permeable), inner (cristae; selectively permeable). Matrix: circular DNA, 70S ribosomes, Krebs cycle enzymes. Inner membrane bears oxysomes (F₁ head = ATP synthase, F₀ = proton channel). Absent in RBCs and prokaryotes.
- Easy : ‘Powerhouse of the cell’ = makes ATP.
- Medium : Cristae = folds of the inner membrane; increase surface area.
- Exam-type (CET) : Having DNA and ribosomes, mitochondria divide on their own.
Rule 2 : Plastids and ribosomes — Leucoplast (colourless; storage: amyloplast starch, elaioplast oil, aleuroplast proteins), chromoplast (carotene, xanthophyll; red/yellow), chloroplast (chlorophyll; grana + stroma + thylakoids; 20–40 in mesophyll). Ribosome: rRNA + protein, Palade 1953; 80S = 60S + 40S; free (enzymes) and bound (secretory proteins); polyribosomes.
- Easy : White potato = leucoplast; red hibiscus petal = chromoplast.
- Medium : Ribbon-shaped in Spirogyra; one large chloroplast in Chlamydomonas.
- Exam-type (CET) : Venkatraman Ramakrishnan: 2009 Nobel (Chemistry) for ribosome structure.
Mitochondria = ATP (oxysomes); chloroplast = food (grana); ribosome = proteins (70S/80S).
‘Ribosome has a membrane’ is wrong. Trap: ‘mitochondria have 80S’ is wrong (70S).
Memory clue : "Mito = ATP machine; Chloro = chlorophyll + grana".
Spot the difference
| Organelle | Membrane | Key |
|---|---|---|
| Mitochondrion | Double | Cristae, oxysomes |
| Chloroplast | Double | Grana, thylakoids |
| Ribosome | None | 70S / 80S |
Practice questions
- 1Why are mitochondria called powerhouses?
- 2What are cristae?
- 3Function of oxysomes?
- 4Three kinds of leucoplast.
- 5Pigments of chromoplast?
- 6Parts of a chloroplast.
- 7Prokaryotic vs eukaryotic ribosomes.
- 8What is the endosymbiont theory?
Show answers (try them yourself first)
- 1They make ATP.
- 2Folds of the inner membrane.
- 3Proton pumping and ATP synthesis.
- 4Amyloplast, elaioplast, aleuroplast.
- 5Carotene, xanthophyll.
- 6Grana, stroma, thylakoids, stromal lamellae.
- 770S (50S+30S) / 80S (60S+40S).
- 8Mitochondria and chloroplasts arose from bacteria living inside cells.
Nucleus, cytoskeleton, cilia and centrioles
A principal runs a school; in a cell this is the nucleus, holding DNA, chromosomes and instructions.
Nucleus = control centre with a double nuclear envelope, nucleoplasm, nucleolus and chromatin. Cytoskeleton = microtubules, microfilaments, intermediate filaments. Cilia/flagella = movement; centriole = 9 triplets.
In the figure : Nucleus, the 9+2 structure of cilia/flagella, centriole and cytoskeleton.
Rule 1 : Nucleus — Nuclear envelope: two membranes, perinuclear space (10–50 nm), nucleopores. Outer membrane joins ER; inner lined by nuclear lamina. Nucleoplasm (karyolymph) has chromatin (DNA + histones) and nucleolus (rRNA + ribosomal proteins; makes ribosomes). Euchromatin = active; heterochromatin = inert. Human cells have 46 chromosomes.
- Easy : The nucleolus has no membrane.
- Medium : Nucleopores regulate exchange of molecules.
- Exam-type (CET) : Robert Brown (1881) discovered the nucleus.
Rule 2 : Cytoskeleton, cilia/flagella and centrioles — Microtubules (tubulin), microfilaments (actin), intermediate filaments → shape, motility, division. Cilia: short, many, oar-like; flagella: long, few. Structure: basal body (from centriole), basal plate, shaft = axoneme 9+2 (9 doublets + 2 central), radial spokes, sheath. Centrosome: 2 centrioles at right angles; each with 9 triplets, cartwheel; forms spindle and gives basal bodies.
- Easy : 9+2 = cilia/flagella; 9 triplets = centriole/basal body.
- Medium : Centrosome lies near the nucleus in animal cells.
- Exam-type (CET) : A bacterial flagellum differs structurally from a eukaryotic one.
Nucleus = DNA + nucleolus; cilia/flagella = 9+2; centriole = 9 triplets.
‘Centriole = 9+2’ is wrong (9 triplets). Trap: ‘nucleolus has a membrane’ is wrong.
Memory clue : "Cilia = 9+2; centriole = 9 triplets (3×9)".
Spot the difference
| Structure | Arrangement | Function |
|---|---|---|
| Cilia / flagella | 9 + 2 | Movement |
| Centriole | 9 triplets | Spindle, basal body |
| Nucleolus | No membrane | Ribosome biogenesis |
Practice questions
- 1Function of nucleolus?
- 2Function of nucleopores?
- 3Euchromatin vs heterochromatin.
- 4Three parts of the cytoskeleton.
- 5Cilia vs flagella.
- 6What does 9+2 mean?
- 7Structure of a centriole.
- 8Function of centrosome?
Show answers (try them yourself first)
- 1Makes rRNA and ribosomes.
- 2Exchange between nucleus and cytoplasm.
- 3Active, extended / inert, condensed.
- 4Microtubules, microfilaments, intermediate filaments.
- 5Cilia short and many; flagella long and few.
- 69 peripheral doublets and 2 central fibrils.
- 79 triplet microtubules; cartwheel.
- 8Forms spindle; gives basal bodies.
Quick revision of the whole lesson
| Concept | Key fact |
|---|---|
| Cell theory | Hooke 1665 → Schleiden 1838 → Schwann 1839 → Virchow 1855 |
| Prokaryote | 70S, nucleoid, plasmid, mesosome |
| Membrane / wall | Fluid mosaic 1972; ML → PW → SW |
| ER / Golgi | RER proteins; SER lipids; Golgi cis → trans |
| Lysosome / vacuole | Digestion, suicide bag; tonoplast |
| Mito / plastid / ribosome | ATP; grana; 70S vs 80S |
| Nucleus & beyond | Nucleolus; 9+2; centriole = 9 triplets |
🎯 Most important points for the exam
- Timeline of the cell theory.
- Prokaryote vs eukaryote.
- Fluid mosaic model and transport types.
- Functions of ER, Golgi and lysosome.
- Mitochondria / chloroplast structure, 70S–80S.
- Cilia (9+2) and centriole (9 triplets).
Understand the concept → draw the figure → memorise the key points → solve questions yourself.