Plant breeding and the Green Revolution
Last lesson covered disease and health; here we learn how living things help raise food production for a growing population.
A farmer keeps the best grains as next season's seed — the start of selection.
Plant breeding = scientific method of creating new varieties with useful traits. A variety = crop line with uniform traits.
In the figure : Breeding steps and varieties.
Rule 1 : Steps of plant breeding — (1) Collect variation — wild/local lines; germplasm (gene bank). (2) Selection — plants with wanted traits. (3) Hybridisation — cross two parents; combine traits. (4) Selection again among hybrids. (5) Testing — many sites, many seasons. (6) Release — multiply seed for farmers. Aims: higher yield, disease/pest resistance, better quality, drought/salt tolerance.
- Easy : Germplasm = gene bank.
- Medium : Selection twice.
- Exam-type (CET) : No variety without testing.
Rule 2 : Green Revolution and varieties — Norman Borlaug (Nobel 1970) developed dwarf, high-yield wheat; in India M. S. Swaminathan helped adopt them. Wheat: Sonalika, Kalyan Sona; rice: IR-8, Jaya. Resistant: Himgiri (wheat — rust), Pusa Swarnim (mustard — fungal), Pusa Shubra (cauliflower), Pusa Sawani (okra — yellow mosaic virus). Being dwarf, plants do not fall over even with heavy grain.
- Easy : Borlaug = dwarf wheat.
- Medium : Pusa Sawani = okra.
- Exam-type (CET) : Himgiri = rust-resistant.
Collect → select → cross → test → release.
‘Sonalika is a rice variety’ is wrong (wheat). Trap: ‘Pusa Sawani is mustard’ is wrong (okra).
Memory clue : "Sonalika, Kalyan Sona = golden wheat".
Spot the difference
| Crop | Variety |
|---|---|
| Wheat | Sonalika, Himgiri |
| Rice | IR-8, Jaya |
| Okra | Pusa Sawani |
Practice questions
- 1What is germplasm?
- 2Father of Green Revolution?
- 3Benefit of dwarf wheat?
- 4Himgiri resists?
- 5Pusa Shubra is?
- 6Rice varieties?
- 7Hybridisation means?
- 8Why testing?
Show answers (try them yourself first)
- 1Gene bank / genetic diversity.
- 2Norman Borlaug.
- 3Plants do not lodge.
- 4Rust disease.
- 5A cauliflower variety.
- 6IR-8, Jaya.
- 7Crossing two parents.
- 8To confirm stable performance.
Mutation, polyploidy and tissue culture
A single leaf in a pot can sprout a new plant — a cell's ability to rebuild a whole plant.
Totipotency = ability of a single cell to form a whole organism. Tissue culture = growing plants from cells/tissues on artificial medium.
In the figure : Mutation, polyploidy, tissue culture.
Rule 1 : Mutation and polyploidy — Mutation breeding: γ-rays or chemicals change genes; useful changes are selected (e.g. disease-resistant lines). Polyploidy: more chromosome sets (with colchicine) — bigger fruits/grains. E.g. Triticale = wheat × rye (wheat quality + rye hardiness). Haploid plants through anther culture; doubled to give homozygous pure lines.
- Easy : Colchicine = polyploidy.
- Medium : Triticale = wheat × rye.
- Exam-type (CET) : γ-rays = mutation.
Rule 2 : Tissue culture — Explant (leaf/root/shoot tip) → sterile MS medium (mineral salts, sucrose, auxin, cytokinin) → callus (undifferentiated cells) → organogenesis (roots/shoots) → plantlet → hardening → field. More auxin ⇒ roots; more cytokinin ⇒ shoots. Uses: micropropagation (rapid), meristem culture (virus-free plants), somaclones (identical), all-year, small space. E.g. banana, sugarcane, orchid, potato.
- Easy : Callus = undifferentiated.
- Medium : Meristem = virus-free.
- Exam-type (CET) : Auxin → roots.
Auxin → anchor roots; cytokinin → shoots.
‘Callus is differentiated’ is wrong. Trap: ‘colchicine mutates genes’ is wrong (doubles chromosome sets).
Memory clue : "Totipotent = total potential".
Spot the difference
| Method | Use |
|---|---|
| Meristem culture | Virus-free plant |
| Colchicine | Polyploidy |
| Anther culture | Haploid |
Practice questions
- 1Totipotency?
- 2What is callus?
- 3High auxin gives?
- 4Benefit of meristem culture?
- 5Triticale is?
- 6Colchicine used for?
- 7What is hardening?
- 8MS medium?
Show answers (try them yourself first)
- 1Whole plant from one cell.
- 2Mass of undifferentiated cells.
- 3Roots.
- 4Virus-free plants.
- 5Wheat × rye.
- 6Polyploidy.
- 7Acclimatising plantlets outdoors.
- 8Murashige–Skoog nutrient medium.
SCP and biofortification
Microbes in curd and dosa batter are part of food — some microbes become protein food directly.
SCP (single-cell protein) = protein-rich food obtained from microbes. Biofortification = increasing nutrient content of crops.
In the figure : SCP and biofortification.
Rule 1 : Single Cell Protein — Microbes: Spirulina (alga), Chlorella, Aspergillus, Saccharomyces, Methylophilus. Merits: quick growth, 50–60% protein, uses waste (potato-processing waste, straw) as medium, small space, eco-friendly. Limits: safety testing, cost, taste. Uses: human food supplement, animal feed.
- Easy : Spirulina = algal SCP.
- Medium : Protein 50–60%.
- Exam-type (CET) : Grows on waste.
Rule 2 : Biofortification — Raise protein, vitamins, minerals in crops by breeding or genetic engineering. E.g. Atlas 66 (high-protein wheat), iron-rich rice/pearl millet, vitamin A-rich carrot, spinach, pumpkin. Aim: reduce hidden hunger (micronutrient deficiency despite full stomach); helps malnourished children. Methods: conventional breeding, genetic engineering (e.g. golden rice).
- Easy : Atlas 66 = protein.
- Medium : Hidden hunger.
- Exam-type (CET) : Golden rice = vitamin A.
SCP = tiny protein factories.
‘SCP is protein from one animal’ is wrong. Trap: ‘biofortification = using fertilisers’ is wrong.
Memory clue : "Bio-FORTify = fortress of nutrients in crops".
Spot the difference
| Concept | Example |
|---|---|
| SCP | Spirulina |
| Biofortified | Atlas 66 |
Practice questions
- 1SCP stands for?
- 2An SCP organism?
- 3Protein % in SCP?
- 4Atlas 66 is?
- 5Hidden hunger?
- 6Golden rice rich in?
- 7SCP grows on?
- 8A biofortification method?
Show answers (try them yourself first)
- 1Single Cell Protein.
- 2Spirulina.
- 3About 50–60%.
- 4High-protein wheat.
- 5Micronutrient deficiency.
- 6Vitamin A.
- 7Waste materials.
- 8Breeding / genetic engineering.
Animal husbandry and allied trades
Packet milk, a breakfast egg, honey on bread — all from animal husbandry.
Animal husbandry = care of animals — feed, shelter, breeding, health. A breed = group of animals with uniform traits.
In the figure : Dairy, poultry, apiculture, fisheries.
Rule 1 : Dairy, poultry, bees and fish — Dairy: indigenous — Sahiwal, Gir; exotic — Jersey, Holstein; buffalo — Murrah; White Revolution (Operation Flood): Verghese Kurien. Poultry: Leghorn (best layer), Rhode Island Red; eggs and meat. Apiculture: Apis cerana indica; honey, wax; improves pollination; hive management. Fisheries: freshwater (rohu, catla) and marine (pomfret, mackerel); Blue Revolution.
- Easy : Kurien = White Revolution.
- Medium : Leghorn = eggs.
- Exam-type (CET) : Bees aid pollination.
Rule 2 : Breeding, AI and MOET — Inbreeding: closely related mates; pure traits, but inbreeding depression. Outcrossing: unrelated animals of the same breed; cross-breeding: two different breeds — e.g. Hisardale (Bikaneri ewe × Merino ram). AI (artificial insemination): frozen semen of a superior male used for many females. MOET (Multiple Ovulation Embryo Transfer): hormones trigger many eggs, fertilised, embryos placed in other females; more calves from a superior cow.
- Easy : Hisardale = cross-breed.
- Medium : AI = frozen semen.
- Exam-type (CET) : MOET = embryo transfer.
MOET = many eggs, many calves.
‘Jersey is indigenous’ is wrong (exotic). Trap: ‘AI is fertilisation outside the body’ is wrong (semen introduced into female).
Memory clue : "Kurien → milk; blue → fish".
Spot the difference
| Breeding | Meaning |
|---|---|
| Inbreeding | Related mates |
| Outcrossing | Unrelated, same breed |
| Cross-breeding | Different breeds |
Practice questions
- 1An indigenous cow breed?
- 2A buffalo breed?
- 3Best layer?
- 4Leader of White Revolution?
- 5Indian bee species?
- 6Hisardale is?
- 7AI means?
- 8MOET gives?
Show answers (try them yourself first)
- 1Sahiwal / Gir.
- 2Murrah.
- 3Leghorn.
- 4Verghese Kurien.
- 5Apis cerana indica.
- 6Bikaneri ewe × Merino ram.
- 7Artificial insemination.
- 8Many embryos from one female.
Industrial microbes
Curd, idli batter and rising bread — everyday microbial industries.
Fermentation = microbes convert materials into useful products. BOD = oxygen needed to break down organic matter in water.
In the figure : Industrial microbes.
Rule 1 : Foods, drugs, chemicals — Curd: Lactobacillus turns lactose into lactic acid. Idli/dosa/bread: Saccharomyces ⇒ CO₂ ⇒ rising. Swiss cheese: Propionibacterium (CO₂ holes). Penicillin: Penicillium notatum (Fleming). Streptokinase: Streptococcus (dissolves blood clots). Cyclosporin A: Trichoderma (immunosuppressant in transplants). Statins: Monascus (lowers cholesterol). Citric acid: Aspergillus niger; acetic acid: Acetobacter; lactic acid: Lactobacillus. Beverages and biodiesel also by fermentation.
- Easy : Penicillin: Penicillium.
- Medium : Statin: Monascus.
- Exam-type (CET) : Citric: Aspergillus.
Rule 2 : How fermentation is used — A fermenter (bioreactor) controls temperature, pH, oxygen; selected microbes and nutrient medium. The product is separated and purified (downstream processing). Merits: fast, cheap, renewable raw material. E.g. antibiotics, enzymes, organic acids, lipase (detergents), pectinase (clearing juice).
- Easy : Fermenter = controlled tank.
- Medium : Downstream = purification.
- Exam-type (CET) : Lipase = detergents.
Fleming → Penicillium, a fungus.
‘Penicillin from Streptococcus’ is wrong. Trap: ‘statins from Aspergillus’ is wrong (Monascus).
Memory clue : "Fleming → Penicillium, a fungus".
Spot the difference
| Product | Microbe |
|---|---|
| Penicillin | Penicillium |
| Citric acid | Aspergillus niger |
| Streptokinase | Streptococcus |
Practice questions
- 1Curd bacterium?
- 2Holes in Swiss cheese due to?
- 3Penicillin source?
- 4Streptokinase use?
- 5Statin source?
- 6Cyclosporin A source?
- 7Citric acid made by?
- 8Fermenter is?
Show answers (try them yourself first)
- 1Lactobacillus.
- 2Propionibacterium CO₂.
- 3Penicillium notatum.
- 4Dissolves clots.
- 5Monascus.
- 6Trichoderma.
- 7Aspergillus niger.
- 8Controlled fermentation vessel.
Sewage treatment and biogas
Cleaning drain water before it reaches the river — the city's biological wash.
Sewage = domestic/industrial waste water. BOD = oxygen needed to break down organic matter in water. Biogas = methane-rich gas from dung/waste.
In the figure : Sewage treatment and biogas.
Rule 1 : Sewage treatment — Primary: screening, sedimentation — removes solids. Secondary (biological): bacteria form flocs in aeration tanks and digest organic matter ⇒ BOD falls; sludge goes to an anaerobic sludge digester ⇒ biogas. Tertiary: disinfection. Biogas: Methanobacterium and other anaerobic bacteria on dung/waste ⇒ methane 50–70%, CO₂, H₂; fuel and electricity; slurry used as manure. In India ‘gobar gas’ (IARI, KVIC).
- Easy : Flocs = bacterial clumps.
- Medium : Low BOD = cleaner.
- Exam-type (CET) : Methane 50–70%.
Rule 2 : Biogas — Dung, farm waste and kitchen waste decay without air (anaerobic); Methanobacterium ⇒ methane (50–70%), CO₂, H₂, a little H₂S. Uses: cooking gas, lamps, electricity; leftover slurry is good organic manure. Merits: clean fuel, waste disposal, less tree-felling. In India ‘gobar gas’ plants (KVIC, IARI).
- Easy : Anaerobic = without air.
- Medium : Slurry = manure.
- Exam-type (CET) : Methane = fuel.
P-S-T: solids, biological, disinfection.
‘High BOD = clean water’ is wrong. Trap: ‘biogas is mostly CO₂’ is wrong (methane).
Memory clue : "Primary picks solids; secondary bacteria digest".
Spot the difference
| Stage | Job |
|---|---|
| Primary | Remove solids |
| Secondary | Biological digestion |
| Tertiary | Disinfection |
Practice questions
- 1What happens in primary?
- 2Flocs are?
- 3Low BOD means?
- 4Sludge goes to?
- 5Main gas in biogas?
- 6Biogas bacterium?
- 7Use of slurry?
- 8Tertiary treatment?
Show answers (try them yourself first)
- 1Screening, sedimentation.
- 2Clumps of bacteria.
- 3Cleaner water.
- 4Anaerobic digester.
- 5Methane.
- 6Methanobacterium.
- 7Organic manure.
- 8Disinfection.
Biocontrol and biofertilisers
A ladybird in the garden eats aphids — pest control without spraying.
Biocontrol = using living organisms to control pests/diseases. A biofertiliser = living microbe that improves soil fertility.
In the figure : Biocontrol and biofertilisers.
Rule 1 : Biocontrol — Ladybird (eats aphids), dragonfly (mosquitoes), Bacillus thuringiensis (Bt) — toxin kills caterpillars; Trichoderma — root fungal diseases; baculovirus — specific insects, harmless to others. IPM (Integrated Pest Management): biological + cultural + minimal chemicals. Merits: no pesticide residues, beneficial insects survive, eco-friendly.
- Easy : Bt = kills caterpillars.
- Medium : Ladybird = aphid.
- Exam-type (CET) : IPM = integrated.
Rule 2 : Biofertilisers — Rhizobium: fixes N₂ in legume root nodules; Azotobacter, Azospirillum: free-living bacteria; cyanobacteria (Nostoc, Anabaena) and Azolla + Anabaena: nitrogen in rice fields; mycorrhiza (Glomus): fungus–root symbiosis, absorbs phosphorus and water; PSB: phosphate-solubilising bacteria. Merits: nitrogen/phosphorus available, less chemical fertiliser, improved soil. A biofertiliser is living — not a chemical.
- Easy : Rhizobium = legumes.
- Medium : Azolla = rice.
- Exam-type (CET) : Mycorrhiza = phosphorus.
R–L: Rhizobium–legume; A–R: Azolla–rice.
‘Biofertiliser = urea’ is wrong. Trap: ‘Rhizobium in rice’ is wrong (legumes).
Memory clue : "Bt kills caterpillars, protects crops".
Spot the difference
| Organism | Use |
|---|---|
| Rhizobium | N₂ fixation |
| Mycorrhiza | Phosphorus uptake |
| Trichoderma | Fungal control |
Practice questions
- 1Ladybird eats?
- 2Bt expands to?
- 3Baculovirus acts on?
- 4IPM stands for?
- 5Rhizobium lives in?
- 6In rice fields?
- 7Mycorrhiza helps absorb?
- 8PSB?
Show answers (try them yourself first)
- 1Aphids.
- 2Bacillus thuringiensis.
- 3Specific insects.
- 4Integrated Pest Management.
- 5Legume root nodules.
- 6Azolla + Anabaena.
- 7Phosphorus.
- 8Phosphate-solubilising bacteria.
Quick revision of the whole lesson
| Concept | Key fact |
|---|---|
| Plant breeding | Selection, crossing, testing; Borlaug |
| Tissue culture | Callus; auxin–cytokinin; meristem |
| SCP | Spirulina; 50–60% protein |
| Animal husbandry | Kurien; Leghorn; AI; MOET |
| Industrial | Penicillium; Monascus; biogas |
| Biocontrol | Bt, ladybird, Trichoderma |
| Biofertilisers | Rhizobium, Azolla, mycorrhiza |
🎯 Most important points for the exam
- Plant breeding and the Green Revolution.
- Mutation, polyploidy, tissue culture.
- SCP and biofortification.
- Animal husbandry and allied trades.
- Industrial microbes, sewage, biogas.
- Biocontrol and biofertilisers.
- Summary of varieties and examples.
Understand the concept → draw the figure → memorise the key points → solve questions yourself.